Repair of UV-induced (6–4)photoproducts measured in individual genes in the Drosophila embryonic Kc cell line
de Cock, J.G.R.; van Hoffen, A.; Wijnands, J.; Molenaar, G.; Lohman, P.H.M.; Eeken, J.C.J.; de Cock J.G.R.; MGC-Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden; J.H.Cohen Institute, Interuniversity Research Institute for Radiopathology and Radiation Protection; van Hoffen A.; MGC-Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden; Wijnands J.; MGC-Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden; Molenaar G.; Department of Biochemistry, State University State University of Leiden; Lohman P.H.M.; MGC-Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden; J.H.Cohen Institute, Interuniversity Research Institute for Radiopathology and Radiation Protection; Eeken J.C.J.; MGC-Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden; J.H.Cohen Institute, Interuniversity Research Institute for Radiopathology and Radiation Protection
Журнал:
Nucleic Acids Research
Дата:
1992
Аннотация:
The nucleotide excision repair (NER; dark-repair) of (6–4)photoproducts ((6–4)PPs) was assayed in cells from a permanent Drosophila melanogaster embryonic cell line, Kc, after exposure to 20 or 40 J/m<sup>2</sup> ultraviolet (UV) light. Induction rates in the transcriptionally active genes Gart and Notch as well as In the inactive white locus is similar. They are formed with a frequency of about one-third of that of cyclobutane pyrimidine dimers (CPDs). In all three genes, (6–4)PPs are repaired with the same rate and to the same extent: 31% of the (6–4)PPs are removed in 4 hours post-Irradiation and after 16 hours repair is nearly complete. In none of the three genes strand-specific repair was found. Exposure of cells that were irradiated with 40 J/m<sup>2</sup> UV to photoreactivating light for 1 hour prior to dark-repair Incubation, resulted in enhanced repair of (6–4)PP8.
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